Antagonistic Activity of Lactic Acid Bacteria Against Pathogenic Vibrios and Their Potential Use as Probiotics in Shrimp (Penaeus vannamei) Culture

Probiotic use in aquaculture settings can be an approach for disease control and dietary supplementation. We assessed the antagonistic effect of culture supernatants lactic acid bacteria on growth known shrimp pathogens, Vibrio ( Listonella ) anguillarum , alginolyticus V. harveyi using a quantitative microplate bioassay. Supernatants from Lactobacillus curvatus subsp. curvatus, L. plantarum Pediococcus acidolactici significantly inhibited these vibrios. The active component(s) were heat stable (> 100°C) resistant to freeze-thawing. Most this inhibitory activity was brought about by production pH; however, there evidence other factors playing role. In search novel probiotic bacteria, organism isolated gastrointestinal tract healthy whiteleg Penaeus vannamei)— identified tentatively as Carnobacterium maltaromaticum . This isolate, had less potent vibriocidal than reduced survival at dose 1 × 10 7 bacteria/shrimp. During 28-day feeding trial, juvenile P. vannamei fed with supplemented diets showed no gross changes parameters compared control. suggest that could incorporated into biofloc formulations purge pathogenic vibrios pond settings, rather being directly shrimp.